This Mouse Plexin C1 ELISA Kit from Innovative Research is intended for quantitative detection of mouse Plexin C1 in cell culture supernates, serum and plasma (heparin, EDTA). Strip well format. Reagents for up to 96 tests.
This mouse Plexin C1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Plexin C1 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: A35-T950) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Plexin C1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Fibronectin amount of sample captured in plate.
- Detection Target: Plexin C1
- Uniprot ID: )
- Reactivity: Mouse
- Cross-Reactivity: There is no detectable cross-reactivity with other relevant proteins.
- Range: 93.8pg/ml-6000pg/ml
- Sensitivity: <50pg/ml
- Storage Conditions: Store at 4?C for 6 months, at -20?C for 12 months. Avoid multiple freeze-thaw cycles. (Shipped with wet ice)
Additional Information: The capture antibody is a monoclonal antibody from rat, the detection antibody is a biotinylated detection polyclonal antibody from goat. Expression system for standard: A Plexin is a protein which acts as a receptor for Semaphorin family signaling proteins. This gene is mapped to 10; 10 C2. It is classically known for its expression on the surface of axon growth cones and involvement in signal transduction to steer axon growth away from the source of Semaphorin. Plexin also has implications in development of other body systems by activating GTPase enzymes to induce a number of intracellular biochemical changes leading to a variety of downstream effects.; Immunogen sequence: 369
